ABSTRACT
Objective:To discuss the influence of Tongfengning Capsule(TFN)in the rats with acute gouty arthritis,and to clarify the curative effect and mechanism of TFN in the acute gouty arthritis rats.Methods:The rat model of acute gouty arthritis was induced by injection of microcrystal sodium urate solution into articular cavity of the ankle joint.A total of 48 mice were divided into blank control group,model group,low(100 mg·kg-1),middle(200 mg·kg-1)and high(550 mg·kg-1)doses of TFN groups,colchicine(0.63 mg·kg-1)positive drug control group;8 rats in each group.The degrees of joint swelling of the rats were monitored at different time after modeling.The number of white blood cells(WBC),lymphocytes and neutrophils in the whole blood of the rats in various groups were detected,and the nitric oxide(NO)levels in the serum and homogenate as well as serum uricacid(UA)were detected.The levels of IL-1βand TNF-αin the soft tissue and synovial fluid around the ankle joint of the rats in various groups were measured.Results:Compared with model group,the swelling degrees of ankle joint of the rats in positive drug control group and high dose of TFN group were significantly decreased in 0-8 h(P<0.05 or P<0.01),especially in 4 h(P<0.01);but there were no significant differences in low and medium doses of TFN groups(P> 0.05).Compared with model group,the levels of WBC, lymphocytes and neutrophils in the blood of the rats in positive drug control group and TFN groups were decreased, especially in high dose of TFN group(P<0.05).The UA levels in serum of the rats in positive drug control group and high dose of TFN group were significantly lower than that in model group(P<0.05 or P<0.01),and the levels of serum NO was increased(P<0.05);but there were no significant differences in low and medium doses of TFN groups(P<0.05).The levels of NO,IL-1βand TNF-αin soft tissue and synovial fluid of the rats in positive drug control group and high dose of TFN group were significantly lower than those in model group(P<0.05 or P< 0.01);but there were no significant differences in low and medium doses of TFN groups(P<0.05). Conclusion:TFN has a remarkable effect in the treatment of gouty arthritis,and this effect may be associated with inhibiting the inflammatory reactions.
ABSTRACT
Objective:To discuss the influence of Tongfengning Capsule (TFN) in the levels of uricacid (UC),creatinine (Cr) and urea nitrogen (BUN) in mouse serum and the activities of the xanthine oxidase (XOD),adenosine deaminase (ADA) activity in the liver homogenate of the mice with hyperuricemia,and to observe the improvement effect of TFN on the pathological changes of liver tissue and to clarify its mechanisms.Methods:The models of mouse hyperuricemia were induced by yeast extract with potassium oxonate.Seventy mice were divided into blank control group,model group,low (200 mg · kg 1),medium (400 mg · kg-1) and high (800 mg · kg-1) doses of TFN groups,allopurinol positive drug control group (50 mg · kg-1),Tongfengshu (TFS,600 mg · kg-1) positive drug control group (n=10).The levels of UC,Cr,BUN in serum and the activities of XOD,ADA in homoggenate were detected and the histopathological changes of the kidney tissue of the mice were measured with HE staining.Results:Compared with blank control group,the levels of serum UC,Cr and BUN ofthe mice in model group were significantlyincreased (P＜0.01),and the activities of XOD and ADA in liver tissue were also increased (P＜0.01).Compared with model group,the levels of serum UC,Cr and BUN of the mice in positive drug control groups and different doses of TFN groups were decreased (P＜0.01),and the activities of XOD and ADA in liver tissue were also decreased (P＜0.05),especially in high dose of TFN group.Compared with model group,the pathologic changes such as renal glomerulus atrophy,renal interstitial fibrosis and expansion of renal tubule of the mice in positive drug control groups and high dose of TFN group were improved to a certain extent.Conclusion:TFN has improvement effcet on the hyperuricemia in the mice and its mechanism is related to the inhibition of uricogenesis and the promotion of UC excretion.
ABSTRACT
Objective:To discuss the influence of Tongfengning Capsule (TFN) in the levels of uricacid (UC),creatinine (Cr) and urea nitrogen (BUN) in mouse serum and the activities of the xanthine oxidase (XOD),adenosine deaminase (ADA) activity in the liver homogenate of the mice with hyperuricemia,and to observe the improvement effect of TFN on the pathological changes of liver tissue and to clarify its mechanisms.Methods:The models of mouse hyperuricemia were induced by yeast extract with potassium oxonate.Seventy mice were divided into blank control group,model group,low (200 mg · kg 1),medium (400 mg · kg-1) and high (800 mg · kg-1) doses of TFN groups,allopurinol positive drug control group (50 mg · kg-1),Tongfengshu (TFS,600 mg · kg-1) positive drug control group (n=10).The levels of UC,Cr,BUN in serum and the activities of XOD,ADA in homoggenate were detected and the histopathological changes of the kidney tissue of the mice were measured with HE staining.Results:Compared with blank control group,the levels of serum UC,Cr and BUN ofthe mice in model group were significantlyincreased (P＜0.01),and the activities of XOD and ADA in liver tissue were also increased (P＜0.01).Compared with model group,the levels of serum UC,Cr and BUN of the mice in positive drug control groups and different doses of TFN groups were decreased (P＜0.01),and the activities of XOD and ADA in liver tissue were also decreased (P＜0.05),especially in high dose of TFN group.Compared with model group,the pathologic changes such as renal glomerulus atrophy,renal interstitial fibrosis and expansion of renal tubule of the mice in positive drug control groups and high dose of TFN group were improved to a certain extent.Conclusion:TFN has improvement effcet on the hyperuricemia in the mice and its mechanism is related to the inhibition of uricogenesis and the promotion of UC excretion.